Rapid Cycle Real-Time PCR - Methods and Applications

Rapid Cycle Real-Time PCR is a powerful technique for nucleic

I Methods.- Housekeeping Genes: A Gold Standard?.- The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia.- Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach.- Measuring Genome Sizes by Absolute Quantification.- II Applications.- Regulation and Development.- Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I.- Quantification of ?Rec-? J? Signal Joint T-Cell Receptor Excision Circle DNA in Patients after Autologous and Allogeneic Stem Cell Transplantation.- Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR.- Quantification of Ikaros Splice Variants by Real-Time PCR.- Methods to Quantify Cytokine Gene Expression by Real-Time PCR.- Oncology.- Profiling Breast Cancer Using Real-Time Quantitative PCR.- HER-2/neu Gene Copy Number Quantified by Real-Time PCR in Cell Lines and Breast Cancer Tissue.- Quantification of Several Components of Fibrinolytic and Matrix Metalloproteinase Systems in Primary Breast Cancer.- A Rapid Cycle Real-Time Quantitative (?-globin PCR for Quantification of Human DNAin Feces.- Quantification of Tumor Load for Follicular Lymphoma by Quantifying BCL2/IGH Using Real-Time Quantitative PCR by LightCycler(TM).- Real-Time Quantification of the AML Rearrangements, AML1-ETO and TEL-AML1, in Acute Leukemia.- Genetics.- Rapid Detection of Gene Duplications in Charcot-Marie-Tooth 1A Disease by SNPGenotyping Using Real-Time PCR.- Cytochrome P450 2D6 Deletion Genotyping Using Derivative Curve Analysis on the LightCycler.- Trisomy 21 Detected by SNP Allele Ratios.- Quantitative Chimerism Analysis by Allele-Specific Real-Time PCR of a 10 bpInsertion/Deletion Polymorphism within the Promotor Region of Factor VIIc.- Microbiology.- Detection of SARS-Coronavirus in the LightCycler by 5?-Nuclease Real-Time RT-PCR.- Normalized Quantitative Rapid-Cycle Real-Time PCR for the Assessment of CMV-DNA Copies.