Cell and Tissue Reaction Engineering

The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products ("products from cells"), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells ("cells as product") for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.

"Today mammalian cell products, primarily monoclonals, cytokines, recombinant glycoproteins and increasingly vaccines dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells for regenerative medicine have been introduced on the market.This book elucidates the interactions between biological as well as biochemical and engineering principles in processes derived from cell cultures. The authors explain the basic characteristics of mammalian cells related to cultivation systems and the consequences on design and operation of bioreactor systems, so that the importance of cell culture technology as well as differences compared to microbial fermentation technology are understood. Further, special cell culture applications, such as insect cell-based recombinant protein production, bioartificial organs and plant cell-based bioprocessing, are presented.This book is of interest to students of biotechnology as well as scientists and industrial users."

Mammalian Cells.- Mammalian Cell Culture Technology: An Emerging Field.- Characteristics of Mammalian Cells and Requirements for Cultivation.- Bioreactors for Mammalian Cells: General Overview.- Special Engineering Aspects.- Bioreactor Design and Scale-Up.- Special Applications.- Insect Cell-Based Recombinant Protein Production.- Bioreactors for Bioartificial Organs.- Plant Cell-Based Bioprocessing.