Working with Stem Cells

The content covered encompass not only the theoretic and methodological aspects for culturing human pluripotent stem cells, but also the establishment of induced pluripotent stem cells and their differentiation into specific cellular subtypes. This book has the most updated methods and technologies in an accessible and clear language, presenting protocols developed and optimized by researchers that use them every day. Stem cell research is a growing field that offers the potential to comprehend mechanisms of neural development and tissue regeneration and maintenance as well as the development-related human diseases and the establishment of novel therapies. Questions of basic biology can also be exploited by this cutting-edge approach.

Chapter 1 Stem Cells: Principles and Applications.- Chapter 2 Human Embryonic Stem Cell Line Derivation.- Chapter 3 Adipose-Derived Mesenchymal Stromal Cells.- Chapter 4 Neural Differentiation of Rodent Neural Progenitor Cells & Isolation and Enrichment of Human Neural Progenitor/Stem Cells.- Chapter 5 Mice Post-Natal Subventricular Zone Neurospheres: Derivation, Culture, Differentiation and Applications.- Chapter 6 Very Small Embryonic-like Stem Cells (VSELs) and their Hematopoietic Specification.- Chapter 7 Neural Crest Stem Cell Cultures: Establishment, Characterization and Potential Use.- Chapter 8 Cancer Stem Cells: Issues with in vitro Expansion and Model Systems.- Chapter 9 Spontaneous Generation of Patient-specific Retinal Pigment Epithelial Cells using induced Pluripotent Stem Cell Technology. This chapter includes the generation of induced Pluripotent Stem Cell (iPSC).- Chapter 10 Differentiation of Human Pluripotent Stem Cells into Cortical Neurons.- Chapter 11 MotorNeuron Differentiation from Pluripotent Stem Cells: Development of the Technique, Synopsis of Protocols, and Summary of Current Applications.- Chapter 12 Derivation of Dopaminergic Neurons from Human Pluripotent Stem Cells Using a Defined System and/or Small Molecules.- Chapter 13 Differentiation of hiPSC-derived Cardiomyocytes.- Chapter 14 Endoderm Differentiation from Human Pluripotent Stem Cells.- Chapter 15 Pancreatic Differentiation from Human Pluripotent Stem Cells.- Chapter 16 Efficient Generation of Skeletal Myogenic Progenitors from Human Pluripotent Stem Cells.- Chapter 17. Genome Editing in Stem Cells.- Chapter 18 Neural Stem Cells: Functional Multipotency and Spinal Cord Injury Research Protocols.- Chapter 19 Stem Cells and Tissue Engineering.- Chapter 20 Scaffolds for Embryonic Stem Cell Growth and Differentiation.