Genetic Transformation Systems in Fungi, Volume 2

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. While Volume 1 provided its readers with an in-depth understanding of how the transformation of DNA is used to understand the genetic basis behind these fungal traits, Volume 2 of this text describes the transformation associated methods, tools, and many relevant elements available to the modern fungal researcher.

Anastomosis and Heterokaryon Formation.- Induction of the Sexual Cycle in Filamentous Ascomycetes.- What Have We Learned by Doing Transformations in Neurospora tetrasperma?.- Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process.- Calculating RIP Mutation in Fungal Genomes Using RIPCAL.- Fungal Transposable Elements.- In vivo Targeted Mutagenesis in Yeast using TaGTEAM.- RNA Silencing in Filamentous Fungi: from Basic to Applications.- RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum.- Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigates.- Expanding the Repertoire of Selectable Markers for Aspergillus Transformation.- Arginase (agaA) as a Fungal Transformation Marker.- Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors.- A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma.- Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi.- Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals.- Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi.- Yeast Cell Electroporation in Droplet-Based Microfluidic Chip.- Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis.- Genetic and Genomic Manipulation in Aspergillus niger.- Genetic Manipulation of Meyerozyma guilliermondii.