Methods in Pulmonary Research

"Methods in Pulmonary Research" presents a comprehensive review of methods used to study physiology and the cell biology of the lung. The book covers the entire range of techniques from those that require cell cultures to those using in vivo experimental models. Up-to-date techniques such as intravital microscopy are presented. Yet standard methods such as classical short circuit techniques used to study tracheal transport are fully covered. This book will be extremely useful for all who work in pulmonary research, yet need a practical guide to incorporate other established methods into their research programs. Thus the book will prove to be a valuable resource for cell biologists who wish to use organs in their research programs as well biological scientists who are moving their research programs into more cell related phenomena.

Airways.- 1 Measurement of lung function in rodents in vivo.- 2 The isolated perfused lung.- 3 Lung explants.- 4 Tracheal preparations.- Vessels.- 5 Intravital microscopy: Airway circulation.- 6 The bronchial circulation.- 7 Segmental vascular resistance and compliance from vascular occlusion.- Edema.- 8 Experimental and clinical measurement of pulmonary edema.- 9 Neurogenic inflammation in the airways: Measurement of microvascular leakage.- 10 Intravital microscopy: Surface lung vessels and interstitial pressure.- 11 Lymphatics.- Airway liquid.- 12 Evaluation of secretory and transport processes which determine the composition of airway surface liquid.- 13 Bronchoalveolar lavage.- 14 Assessment of surfactant function.- Cell culture.- 15 Isolation of type II alveolar epithelial cells.- 16 Endothelial cells.- Histology.- 17 Studying lung ultrastructure.- 18 Autoradiography in the lung.- Further methods.- 19 Application of aerosols.- 20 Cryopreservation of human pulmonary tissues.- Appendix I. Physiological data of various mammalian species.- References.- Appendix II. List of suppliers.