UHPLC in Life Sciences

This book presents a comprehensive overview of UHPLIC and is essential reading to newcomers to field and postgradutes and an essential handbook for experienced users of the technique.

1.1. Trends in HPLC; 1.2. Comparison of Chromatographic Techniques and Supports; 1.3. Constructing Kinetic Plots; 1.4. History of the Kinetic Plot Method; 1.5 Unification of the Isocratic and Gradient Kinetic Plot Method; 1.6. Relation between the Kinetic Performance under Isocratic and Gradient Elution Conditions; 1.7. Influence of the Test Conditions on the Obtained Kinetic-Performance Limit Curve; 1.8. Some Reflections on Recent Trends in Liquid Chromatography using the Kinetic Plot Method; 1.9. Conclusions; Acknowledgements; References; 2.1 UHPLC Instrumentation; 2.2 UHPLC Columns; 3.1 Introduction; 3.2 Qualitative Transfer from HPLC to UHPLC; 3.3 Normative Context for the HPLC to UHPLC Transfer; 3.4 Validation of UHPLC Methods and Equivalence of the HPLC-UHPLC Methods; 3.5 Conclusions; 3.6 References; 4.1 Introduction; 4.2 High throughput and high resolution in HT-UHPLC; 4.3 Limitations of HTLC and HT-UHPLC; 4.4 Advantage of high temperature in life science analysis; 4.5 HT-UHPLC in comprehensive on-line two-dimensional liquid chromatography (LC x LC); 4.6 Conclusion; 5. Comparison of the performance of totally porous and core-shell particles; 5.1. Introduction; 5.2. Column performance; 5.3. Possibilities of recent core-shell technology; 5.4. Particle size distribution and roughness of core-shell particles; 5.5. Loading capacity of core-shell particles; 5.6. Limited efficiency when core-shell particles packed in narrow-bore columns; 5.7. Extra column effects, contribution to band broadening; 5.8. Performance of core-shell and totally porous particles in isocratic elution mode; 5.9. Performance of core-shell and totally porous particles in gradient elution mode; 5.10. Conclusion; 6.1 Introduction; 6.2 Analytical Conditions for performing HILIC.; 6.3 Applications of HILIC in UHPLC.; 6.4 References.; 7.1 Introduction; 7.2 Selection of ionization techniques; 7.3 Overview of mass analyzers and their main features; 7.4 New developments in mass spectrometry applicable in UHPLC/MS; 7.5 Conclusions; 8.1 Introduction; 8.2 Solubility; 8.3 Ionization; 8.4 Lipophilicity; 8.5 Permeability; 8.6 Conclusion; 8.7 References; 9.1 Introduction; 9.2 UHPLC in Bioanalysis; 9.3 Sample preparation for UHPLC in bioanalysis; 9.4 Conclusions; 10.1 Introduction;10.2 GC-MS Analyses; 10.3 LC-MS(/MS) Analyses; 10.4 Application of UHPLC-MS(/MS) for Drug Testing in Sports; 10.5 Conclusion; References; 11.1 Introduction; 11.2 Use of UHPLC for the Analysis of Seized Drugs; 12.1. Pharmaceuticals as environmental contaminants; 12.2. Analysis of pharmaceuticals in environmental samples; 12.3. Occurrence of pharmaceuticals in environmental and wastewater samples; 12.4. Conclusions; Acknowledgements; References; 13.1 Introduction; 13.2 Multiple Facets of UHPLC in NP research; 13.3 Fast Targeted Analysis; 13.4 Fast Non-Targeted Analysis, Fingerprinting, and Metabolomics; 13.5 High-Resolution Profiling and Metabolite ID; 13.6 Conclusion; Acknowledgments; References; 14.1. Introduction; 14.2. Pre-analysis Considerations: Protocol Design, Sample Collection, Storage and Preparation; 14.3. Sample Preparation for Serum and/Plasma; 14.4. Collection and Storage of Urine Samples; 14.5. UHPLC-MS-Based Metabolite Profiling; 14.6. Applications of UHPLC-MS to Human Metabolic Profiling Studies; 14.7 Current Challenges for UHPLC-MS in Global metabolic profiling Studies; 14.8. Conclusions